Air or fuel-air mixture is forced into the cylinders by natural atmospheric pressure upon opening of the inlet valve or valves. The pressure within the cylinder is lowered by the action of the piston moving away from the valves (so as to expand the volume available for incoming air).
In some cases the lowering of the cylinder pressure is enhanced by a combination of the speed of the exhaust gases leaving the cylinder and the closing of the exhaust valve at the appropriate time. A tuned exhaust can help with this but generally only works at a narrow range of engine speeds and hence is most useful in very high performance cars, aircraft and helicopters.